The investigation of signal transmission in brain requires to reproduce/observe physiological events that occur on a wide range of spatiotemporal scales, from e.g. the localized brief (μs) transient associated with a single (sub micron) synaptic event to the complex arithmetic that dendrites use in integrating (μs-ms) multiple localized synaptic inputs (several micrometers apart).
Light microscopy is a fundamental tool in neuroscience offering a sensitive and non-invasive approach to probe and mimic such brain complexity.
The aim of the Wavefront-engineering microscopy team is to develop a specific class of optical techniques based on spatiotemporal engineering of optical wave-fronts obtained by phase modulation solely. This approach has the advantage of minimizing power losses, permitting a quick adaptability of the excitation pattern to the experimental context and allowing a true 3D sculpting of the excitation volumes.
The team activity is organized in three research lines:
See more at: http://neurophotonics.parisdescartes.cnrs.fr
E. Papagiakoumou,V. de Sars, V. Emiliani, D. Oron,
Temporal focusing with spatially modulated excitation
Optics Express, 17, 5391-5401 (2009)
E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani
Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses
Optics Express 16, 22039-22047 (2008).
Lutz, C. T. Otis, V. DeSars, S. Charpak, D. DiGregorio, and V. Emiliani
Holographyc photolysis of caged neurotransmitters
Nature Methods 5, 821-827 (2008)
A. Genovesio, T. Ledl, V. Emiliani, W. Parak, M. Coppey-Moisan, and Jean-Christophe Olivo-Marin
Multiple single-particle tracking inmultidimensional microscopy: methods and application to the tracking of quantum dots.
IEEE Transactions on Image Processing 15, 1062-1070 (2006)
C. Goletti, V. Emiliani, S. Schintke, A.M. Frisch, N. Esser and B.O. Fimland
Detection of surface states anisotropies at GaAs(001) (2x4) decapped surface
Physica status solids (b) 242, 2664-2670 (2005)
V. Emiliani, D. Cojoc, E. Ferrari, V. Garbin, C. Durieux, M. Coppey-Moisan, and E. Di Fabrizio
" Wave front engineering for living cells microscopy ",
Optics Express 13, 1395-1405 (2005)
E. Di Fabrizio, D. Cojoc V. Emiliani, M. Coppey-Moisan, S. Cabrini, E. Ferrari, V. Garbin, and M. Altissimo,
"Microscopy of biological sample through advanced diffractive optics from visible to x-ray wavelength regime",
Microscopy research and technique 65, 252-262 (2005)