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Center for Neurogenomics and Cognitive Research
Functional Genomics
De Boelelaan 1085

1081 HV - Amsterdam
Netherlands

+31-(0)20-598 69 28
+31-(0)20-598 69 26


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Dr Ruud F. Toonen
Mechanisms of secretory vesicle transport, capture and fusion
Center for Neurogenomics and Cognitive Research (CNCR) Amsterdam

Research Area

The major aim of my research group is to elucidate the mechanisms that orchestrate the assembly of the presynaptic signal transduction machinery.

We study activity dependent transport, capture and release of secretory vesicles in vitro and in vivo using knockin reporter mice.

We focus on key proteins of the release machinery and their interactors and downstream effectors of second messenger systems like PKC, DAG and calcium. We use optical techniques (TIRFM) in combination with electrophysiology to monitor activity-dependent intrasynaptic trafficking towards the plasma membrane.


Publications
Toonen R.F., Verhage M. (2007):The role of Munc18-1 in vesicle secretion: lonely munc joins SNARE family..Trends in Neurosci. (in press):
Verhage M., Toonen R.F. (2007):Vesicle fusion: merging ideas on fusing membranes..Current Opinion in Cell Biology (in press):
Toonen R.F., Kochubey O., de Wit H., Gulyas-Kovacs A., Konijnenburg B., Sorensen J.B., Klingauf J., Verhage M. (2006):Dissecting docking and tethering of secretory vesicles at the target membrane..EMBO J: 25:3725-3737
Toonen R.F., Wierda K., Sons M.S., de Wit H., Cornelisse L.N., Brussaard A., Plomp J.J., Verhage M. (2006):Munc18-1 expression levels control synapse recovery by regulatiing readily releasable pool size..Proc Natl Acad Sci U S A: 103: 18332-18337

Technical Expertise