MRC Centre for Synaptic Plasticity
School of Medical Sciences University of Bristol
Anatomy Department
University Walk
BS8 1TD - Bristol
United Kingdom

+44(0)1173311944

jon.hanley@bristol.ac.uk

Job opportunities

The control of AMPA receptor trafficking is a crucial element of synaptic plasticity and may also be important in post-ischaemic mechanisms leading to cell death.
Such trafficking is governed by protein-protein interactions with AMPA receptor subunits.
The group studies the calcium signaling and phosphorylation pathways that regulate these interactions using molecular biology, biochemistry and confocal microscopy.
This work will provide important insights into the molecular mechanisms of memory and stroke.

Dennis S.H., Jaafari N., Cimarosti H., Hanley J.G., Henley J.M., and Mellor J.R. (2011). Oxygen/Glucose Deprivation Induces a Reduction in Synaptic AMPA Receptors on Hippocampal CA3 Neurons Mediated by Metabotropic Glutamate Receptor 1 and A₃ Receptors. Journal of Neuroscience. 31:11941–11952.

Nakamura Y., Wood C.L., Patton A.P., Jaafari N., Henley J.M., Mellor J.R., and Hanley J.G. (2011). PICK1 inhibition of the Arp2/3 complex controls dendritic spine size and synaptic plasticity. The EMBO Journal. 30:719-30.

Hanley JG. (2010). Endosomal sorting of AMPA receptors in hippocampal neurons. Biochem Soc Trans. 38: 460-465.

Dixon R.M., Mellor J.R. and Hanley J.G. (2009). PICK1-Mediated Glutamate Receptor Subunit 2 (GluR2) Trafficking Contributes To Cell Death In Oxygen/Glucose Deprived Hippocampal Neurons. Journal of Biological Chemistry, 284 14230-5.

Hanley JG. (2008) AMPA receptor trafficking pathways and links to dendritic spine morphogenesis. Cell Adh Migr, 2 276-82.

Rocca D.L., Martin S., Jenkins E.L. and Hanley J.G. (2008). Inhibition of Arp2/3-mediated actin polymerisation by PICK1 regulates neuronal morphology and AMPA receptor endocytosis. Nature Cell Biology, 10 259-271.

Hanley J.G. (2008). PICK1: a multi-talented modulator of AMPA receptor trafficking. Pharmacology and Therapeutics, 118 152-60.

Hanley J.G. (2007). NSF Binds Calcium to Regulate its Interaction with AMPA Receptor Subunit GluR2. Journal of Neurochemistry, 101 1644-50.

Hanley J.G., and Henley J.M. (2005).  PICK1 is a calcium-sensor for NMDA-induced AMPA receptor trafficking. EMBO Journal, 24 3266-78.

Hanley J.G., Khatri L., Hanson P.I., and Ziff E.B (2002).  NSF ATPase and ab SNAPs disassemble the AMPA receptor-PICK1 complex. Neuron, 34 53-67.

Billups D., Hanley J.G^*., Orme M., Attwell D., and Moss S.J. (2000). GABA_C receptor sensitivity is modulated by interaction with MAP1B. Journal of Neuroscience, 20 8643-8650.

* first equal authorship

Hanley J.G., Jones E.M., and Moss S.J. (2000). GABA receptor rho1 subunit interacts with a novel splice variant of the glycine transporter, GLYT-1. Journal of Biological Chemistry, 275 840-846.

Hanley J.G., Koulen P., Bedford F., Gordon-Weeks P.R., and Moss S.J. (1999). The protein MAP-1B links GABA(C) receptors to the cytoskeleton at retinal synapses. Nature, 397 66-69.

Protein-protein interactions, biochemistry, cell biology, confocal imaging.

Receptor trafficking, actin cytoskeleton, dendritic spines, AMPA receptors.