Slice electrophysiology

Brain slice electrophysiology

Confocal microscopy

Neuroanatomy

• Field and whole-cell electrophysiology of hippocampus
• Sindbis and lentivirus construction and production for in vivo expression of recombinant proteins and siRNAs.
• Alzheimer models hippocampal function analysis

My laboratory utilizes a bottom-up approach based on the generation of genetically modified mice and their parallel analysis at the biochemical, molecular, electrophysiological and behavioral levels. We are currently using the following methodologies:

•    Mouse genetics (brain restricted and inducible mutant strains) and use of recombinant lentiviruses

•    Molecular and cell biology techniques with focus on the analysis of gene expression

•    Electrophysiological studies in brain slices

•    Behavioral studies in mice

More information about our expertise can be found here:

http://in.umh.es/paginas_particulares/barco/what%20methods.html

Electrophysiology (sharp intracellular and extracellular recordings in vitro and in vivo, multi channel recordings in vivo, microdialysis, etc.).

Functional magnetic resonance imaging (fMRI) with blood-oxygen-level dependent (BOLD) contrast.

Concomitant application of fMRI, electrophysiological recordings and electric microstimulation.

Manganese enhanced MRI (MEMRI) for neuronal-tract tracing in vivo and fMRI in freely behaving animals.

We use a multidisciplinary set of aproaches ranging from biochemistry and molecular biology, mouse genetics, expression pattern analysis (in situ hybridization, immunohistochemistry...), differential gene profile analysis (microarrays), in utero gene delivery and a wide variety of in vitro assays (retinal explants, retina-chiasm cocultures, retina-colliculo cocultures...).

path clamp

Ca imaging

caged-Ca

image analysis

Two-photon laser scanning miscroscopy of in vivo prepaprations:

- in vivo calcium imaging of neuronal populations with single-cell resolution

- repeated in vivo imaging dendritic and axonal structures

Electrophysiology in vivo

- single- and multi-unit recordings in the visual and auditory cortex

Intrinsic signal imaging

Brain slice electrophysiology

Calcium imaging

Voltage dye imaging

Confocal microcosopy

FRAP

laser scanning microscopy

time-lapse imaging (intracellular recordings and cellular migration)

CNS histology

primary cell cultures (hippocampal neurons, retinal cells, subventricular zone cells, astrocytes, microglia)

in vivo models of rodent epilepsy

Flow cytometry and cell sorting

Confocal microscopy

Neurosphere primary cultures

Cell phate mapping with transgenic animals

Protemics (DIGE, 2DE)

Histology

Genetically modified animals  

Drosophila

Chromatin immunoprecipitation techniques in neural and other stem cells

3D-electron microscopy, electrophysiology, cellular imaging, molecular biology

Behavioral approaches in mice and rats in the fields of: pain, mood disorders, drug abuse.

Neuroanatomy approaches in rodents (tract-tracing...).

Morphological approaches.

Mice in our facilities: KO: MOR, DOR, KOR, beta2-AR, beta2-nAchR; Floxed mice: BDNF; Reporter mice: CRE-LacZ.

- transgenic mice

- purification of neurons and astrocytes

- electrophysiological recordings

- immunostaining, protein biochemistry

Cell and organotypic culture,
in vitro electroporation technics,
Histology,
Confocal imaging
Time-laps imaging

Transgenesis/Gene targeting
Mouse models/genetics
Neuronal cell biology
Yeast genetics/biology

Neurophysiological techniques: imaging and electrophysiology

Multi-patch recordings

Brain slice recordings

Two-photon imaging

Multi-electrode array (MEA) recordings and analysis

Zebrafish Developmental Biology and Genetics

BioImaging

In vivo cell biology

- neuropharmacology,
- developmental neurobiology,
- neuroanatomy,
- behavioral neurosciences,
- cell biology experimentation.

Molecular Biology, Mouse Transgenesis, Viral Gene Delivery